Grazing and mowing are among the most common grassland management practices, and they can significantly influence the belowground food webs, along with microbial biomass and activity and nutrient balance. The aim of this study was to investigate how different land uses, and therefore the associated management practices occurring along the year, affect soil functions and multifunctionality. To fulfil this aim, in the Matese mountain (Southern Italy), adjacent areas characterised by different land uses were selected: meadow-ME, pasture-PA and forest-FO (as undisturbed control). Soil samples were collected at four times along the year, corresponding to specific management practices: sowing, mowing, five months after mowing and, one year after sowing in ME; at the beginning, after one month of grazing, after six months of grazing and, and after spring-rest grazing in PA. Total S and P content, microbial biomass (dsDNA), and eight enzymatic activities were analyzed to assess soil functions related to C, N, P, and S cycles and decomposition. These functions were then used to assess soil multifunctionality using both the averaging approach (SMF index) and the threshold-based approach. Both individual soil functions and overall multifunctionality showed consistent patterns in PA and FO, with significantly higher values compared to ME. In PA, after six months of grazing, functions related to the C and S cycles, as well as soil multifunctionality, increased (by 38% for the C cycle and 6% for both the S cycle and SMF index) compared to FO. In contrast, in ME, during the mowing, S and P cycle functions and the soil multifunctionality decreased (by 22% for both the S and P cycles and 3% for SMF index) compared to FO. Our findings highlight the importance of adopting sustainable management strategies to enhance soil ecosystem functions and services and to fight ecosystem degradation.