This research aims to evaluate the role of three common wetlands macrophytes, Phragmites australis, Typha latifolia, and Glyceria maxima, in nitrogen removal processes, with a focus on the conditions affecting the emissions of nitrous oxide (N₂O), a potent greenhouse gas. Nutrient and gas fluxes were measured using mesocosms containing the three plant species, along with a control bare sediment, following validated experimental protocols for the analysis of benthic biogeochemical dynamics. The mesocosms were placed in tanks filled with water collected from the field site and maintained under controlled laboratory conditions. Experiments were conducted in summer (biomass peak), autumn (senescence phase) and spring (growth phase), across a NO3- gradient (50, 100, 200, 300, 500, and 800 µM). During each incubation, key physicochemical parameters (water temperature, conductivity, pH, and dissolved oxygen concentration) were measured. Water samples were collected at three time points (initial, intermediate, final) for the analysis of dissolved nitrogen nutrients and gasses. Benthic fluxes were calculated for each experimental condition. Vegetated sediments were found to be more efficient at converting NO₃⁻ to N₂ via denitrification than bare sediments. However, the effect of NO₃⁻ pulsing on denitrification stimulation differed significantly between plant types. It can be hypothesized that Phragmites australis played a more beneficial role due to its greater submerged surface area for biofilm colonization, which facilitated enhanced opportunities for contact between denitrifying bacteria and water column NO3-. N2O analyses are underway and could inform the widespread application of phytoremediation by identifying environmental conditions that maximise N removal while minimising the risk of greenhouse gas emissions.