Nature 4.0 is a conceptual framework describing the integration of advanced digital technologies—such as artificial intelligence (AI), the Internet of Things (IoT), and robotics — into the monitoring, management, and restoration of natural ecosystems. It represents the next phase of conservation and environmental stewardship, enabled by cyber-physical systems. Nature 4.0 envisions a cybernetic ecology, where are tightly interwoven to enable real-time, adaptive, and intelligent environmental governance. The technological components of Nature 4.0 paradigm encompass Smart Sensors, AI and Machine Learning, Edge and Cloud Computing and Cyber-ecological Feedback Loops. In this regard, new opportunities for ecological community are arising to extend in space and time monitoring of ecosystem responses to climate changes and human disturbances. We will present some case studies of advanced monitoring systems for ecological applications.